In the research division Biochemical Engineering at the Institute of Chemical, Environmental and Bioscience Engineering (ICEBE) at TU Wien following paid master / diploma thesis project is available:
Information on the physiological state of cells is crucial for the optimization and control of microbial bioprocesses. Intracellular sensors are able to deliver such information in real-time and non-invasively. The aim of this work is to develop new applications of intracellular fluorescent sensors for bioprocess monitoring of an industrially relevant microorganism. The sensor signals will be compared to alternative methods for cell viability estimation and correlated to process variables, e.g. product concentration. Finally, intracellular sensors will be applied for the control of feed rates in a bioprocess based on industrial sugar waste streams.
You will be carrying out measurements with two different state-of-the-art flow cytometry devices and a fluorescence microplate reader. To analyze and modulate the intracellular sensor signal, different cultivation conditions will be tested with microbial strains. Thereby, you can gather experience with assay development and high throughout microscale cultivation. Furthermore, you will conduct HPLC analyses of the obtained samples. The assay is to be transferred to a bioreactor process in the end. You will carefully evaluate large data sets and will be involved in the development of automated data analysis methods.
You are studying biotechnology, biochemistry, bioprocess engineering or a similar subject. Previous experience with microbial laboratory work is a must, experience with flow cytometry would be appreciated. Moreover, you are team-oriented, motivated with a can do attitude and scientifically curious.
Dates and salary
This work would ideally start in November 2018 and is scheduled for 6 months. A small compensation (€ 1800 = 6 x € 300) is possible.
Interested students please contact:
MSc Peter Sinner, email@example.com
We are looking forward to hearing from you!