Your opportunity to learn hands on about Pichia pastoris metabolic engineering, modular cloning with golden gate, screening of enzymes & strains, flow cytometry and bioreactor cultivation.
Your focus will be on optimizing alcohol dehydrogenase activity in the cytoplasm of Pichia pastoris. Expressing and in vivo screening of different alcohol dehydrogenase constructs. Later characterizing the impact on the metabolism. Optionally confirm the finding with bioreactor cultivations.
Pichia pastoris is a highly popular protein expression host used in biopharmaceutical production and industrial applications. It uses glucose as well as methanol for growth and protein production. By selecting and using additional alcohol oxidizing enzymes we may elevate some of the metabolic burdens the cells have when grown under industry relevant conditions.
Your time will be spent at: Department of Biotechnology
- Expressing several alcohol dehydrogenases in P. pastoris.
- Activity screening with a GFP reporter gene and flow cytometry.
- Cloning of multiple alcohol dehydrogenase expression constructs.
- Growth and adaptation experiments.
- Optional: Bioreactor cultivation
Start date: From November 2017 onwards.
If you are a motivated, ambitious and a proactive Master student and interested in this topic please contact:
Brigitte Gasser, Priv.-Doz. Dipl.-Ing. Dr.
+43 1 47654-79033
Domen Zavec, Msc.
+43 1 47654-79142